KMID : 0624620100430110738
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BMB Reports 2010 Volume.43 No. 11 p.738 ~ p.743
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Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1
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Wang Pingzhang
Xiong Ying Ma Chuan Shi Taiping Ma Dalong
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Abstract
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The c-Jun NH(2)-terminal kinase (JNK) signaling pathway participates in many physiological functions. In the current study we reported the cloning and characterization of five novel JNK2 transcript variants, which were designated as JNK2¥á3, JNK2¥á4, JNK2¥â3, JNK2¥ã1 and JNK2¥ã2, respectively. Among them, JNK2¥á4 and JNK2¥ã2 are potential non-coding RNA because they contain pre-mature stop codons. Both JNK2¥á3 and JNK2¥â3 contain an intact kinase domain, and both encode a protein product of 46 kDa, the same as those of JNK2¥á1 and JNK2¥â1. JNK2¥ã1 contains a disrupted kinase domain and it showed a disable function. When over-expressed in mammalian cells, JNK2¥á3 showed higher activity on AP-1 than that of JNK2¥â3 and JNK2¥ã1. Furthermore, JNK2¥á3 and JNK2¥â3 showed different levels of substrate phosphorylation, although they both could promote the proliferation of 293T cells. Our results further demonstrate that JNK2 isoforms preferentially target different substrates and may regulate the expression of various target genes.
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KEYWORD
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AP-1, Cloning, JNK2, MAPK9, Transcript variant
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